Explanation of classes for membrane organisation search

Class A: Soluble, intracellular, no signal-peptide/no transmembrane domain

• There was no ER signal peptide predicted in this sequence
• There was no alpha-helical transmembrane domain predicted in this sequence This sequence is predicted to be soluble and intracellular

Class B: Soluble, secreted, signal-peptide/no transmembrane domain

• There was an ER signal peptide predicted in this sequence
• There was no alpha-helical transmembrane domain predicted in this sequence
• This sequence is predicted to be secreted from the cell

Class C: Type I membrane protein, signal-peptide/one transmembrane domain

• There was an ER signal peptide predicted in this sequence
• There was one alpha-helical transmembrane domain predicted in this sequence
• This sequence is predicted to be a type I membrane protein (N-term out, C-term in)

Class D: Type II membrane protein, no signal-peptide/one transmembrane domain

• There was no ER signal peptide predicted in this sequence
• There was one alpha-helical transmembrane domain predicted in this sequence
• This sequence is predicted to be a type II membrane protein (N-term in, C-term out)

Class E: Multispan membrane protein, >1 transmembrane domain

• There may or may not be an ER signal peptide predicted in this sequence
• There was more than one alpha-helical transmembrane domain predicted in this sequence
• This sequence is predicted to be a type I membrane protein (N-term out, C-term in)

Class F: Unclassified due to prediction conflict

• There was no clear consensus in the predictions for signal peptide and/or transmembrane domains in this sequence

WARNING - alpha-helical transmembrane domains can be mispredicted as signal peptides and vice versa.

This page was last updated on: Friday March 17, 2006